A Resonance Assignment Method for Oriented-Sample Solid-State NMR of Proteins
نویسندگان
چکیده
منابع مشابه
13C spin dilution for simplified and complete solid-state NMR resonance assignment of insoluble biological assemblies.
A strategy for simplified and complete resonance assignment of insoluble and noncrystalline proteins by solid-state NMR (ssNMR) spectroscopy is presented. Proteins produced with [1-(13)C]- or [2-(13)C]glucose are very sparsely labeled, and the resulting 2D ssNMR spectra exhibit smaller line widths (by a factor of ∼2 relative to uniformly labeled proteins) and contain a reduced number of cross-p...
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The overview of Autolink. The overview of Autolink is shown in Figure 1. The input data are spin systems obtained from CARA [2]. All possible spin system parings are considered and a “fitness” score is computed for each pair. A pair of spin systems and their fitness score form a “link hypothesis”. Each fitness score consists of several sub-scores representing specific resonance comparisons, suc...
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ژورنال
عنوان ژورنال: Journal of the American Chemical Society
سال: 2010
ISSN: 0002-7863,1520-5126
DOI: 10.1021/ja102932n